The long-term economic viability of the grapevine industry relies on healthy planting material and effective disease management strategies in vineyards. Grapevine trunk diseases (GTDs) are considered one of the main biotic factors limiting both yield and vineyards’ lifespan worldwide (Gramaje et al. 2018). Studies conducted by the Plant Pathology laboratory at the Summerland Research and Development Centre (SuRDC) since 2010 have significantly contributed to a better understanding of the current status of GTDs in British Columbia (BC), laying the foundation for the development of effective control strategies (O’Gorman et al. 2015; Úrbez-Torres et al. 2014a, 2014b, 2015). However, contrary to most grape-growing countries around the world, neither cultural practices nor registered products (chemical or biological) are currently available to control GTDs in Canada. Therefore, the main objective of this research project was to develop and implement sustainable management strategies against GTDs in both young and mature vineyards as well as at the nursery level. This project investigated cultural practices such as best pruning time to reduce GTDs infection and also focused on potential environment-friendly biological control options. Additionally, this research aimed to demystify the role that abiotic and/or biotic stress factors, such as water stress and nematodes play on GTDs development in young vineyards. The ultimate goal of these studies aims to provide scientific-based information to Canadian growers about best planting and growing conditions to minimize the impact of GTDs.